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مرتع 2022, 16(1): 108-123 |
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Baghari Z, Forouzeh M R, Mazandarani M, Shahiri H, Atashi S. Investigation on changes in some quantitative, morphological and physiological parameters of (Verbascum thapsus) in various elevation classes in Hezar Jarib. مرتع 2022; 16 (1) :108-123
URL: http://rangelandsrm.ir/article-1-950-en.html
URL: http://rangelandsrm.ir/article-1-950-en.html
Department of Rangeland Sciences, Faculty of Rangeland, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan
Abstract: (2304 Views)
Background and purpose: Identifying local medicinal plants being adapted to the climate of Iran, studying the possibility of their production and processing on a large scale, is one of the first steps can be taken for optimal use of such plants. Therefore, in this study, Verbascum thapsus was investigated.
Methodology: The aim of this study is to study the ecological, morphological and phytochemical changes of this plant in four altitude ranges of 1300- 1700- 2100- 2500 with the same slope and direction. Accordingly, after collecting leaves (three repetitions of each plant) of Verbascum thapsus and drying in the laboratory, the plant extract was extracted using soaking method then phytochemical properties such as total phenol, total flavonoids, mucilage, saponin, and antioxidant activity were measured according to standard methods. Also, density changes, canopy cover, production of each plant based on grams per square meter, regeneration (measuring the number of young seedlings per unit area), diameter and leaf length and width of species at different heights were also evaluated.
Results: The results showed that altitude had significant effects (P<0.01) on phenolic, flavonoid, mucilage and antioxidant activity. So that The highest amount of total phenol (116.28 mg gallic acid per gram of dry weight) and total flavonoids (13.24 mg quercetin per gram of dry weight) at an altitude of 2500 m, antioxidant activity by DPPH method (45.31%) at an altitude of 2100 meters, antioxidant activity by IC50 method (82.47 mg / ml) at an altitude of 2500 meters, The highest percentage of mucilage was observed at an altitude of 1700 m, but did not show a significant effect on the amount of saponin in Verbascum thapsus (P>0.05). Regarding ecological characteristics, there is a significant difference among all ecological traits measured except the length and width of leaves at the level of 5% (P<0.05). According to these results, the highest density, at a value of 0.63 base in square meter at an altitude of 2500 meters, the highest percentage of coverage, 9.83 percent at an altitude of 1300 meters, the highest production, 19.43 at an altitude of 2500 meters, the highest reproduction, 0.4 number per square meter at an altitude of 2100 meters, The highest collar diameter, 9.4 cm at a height of 1300 meters was observed.
Conclusion: The results of this study indicate that the altitude factor is effective on some morphological and physiological characteristics of this plant. If to harvest a higher percentage of phenolic, flavonoid and antioxidant compounds is considered, collection of this plant from an altitude of 1700 meters is highly recommended.
Methodology: The aim of this study is to study the ecological, morphological and phytochemical changes of this plant in four altitude ranges of 1300- 1700- 2100- 2500 with the same slope and direction. Accordingly, after collecting leaves (three repetitions of each plant) of Verbascum thapsus and drying in the laboratory, the plant extract was extracted using soaking method then phytochemical properties such as total phenol, total flavonoids, mucilage, saponin, and antioxidant activity were measured according to standard methods. Also, density changes, canopy cover, production of each plant based on grams per square meter, regeneration (measuring the number of young seedlings per unit area), diameter and leaf length and width of species at different heights were also evaluated.
Results: The results showed that altitude had significant effects (P<0.01) on phenolic, flavonoid, mucilage and antioxidant activity. So that The highest amount of total phenol (116.28 mg gallic acid per gram of dry weight) and total flavonoids (13.24 mg quercetin per gram of dry weight) at an altitude of 2500 m, antioxidant activity by DPPH method (45.31%) at an altitude of 2100 meters, antioxidant activity by IC50 method (82.47 mg / ml) at an altitude of 2500 meters, The highest percentage of mucilage was observed at an altitude of 1700 m, but did not show a significant effect on the amount of saponin in Verbascum thapsus (P>0.05). Regarding ecological characteristics, there is a significant difference among all ecological traits measured except the length and width of leaves at the level of 5% (P<0.05). According to these results, the highest density, at a value of 0.63 base in square meter at an altitude of 2500 meters, the highest percentage of coverage, 9.83 percent at an altitude of 1300 meters, the highest production, 19.43 at an altitude of 2500 meters, the highest reproduction, 0.4 number per square meter at an altitude of 2100 meters, The highest collar diameter, 9.4 cm at a height of 1300 meters was observed.
Conclusion: The results of this study indicate that the altitude factor is effective on some morphological and physiological characteristics of this plant. If to harvest a higher percentage of phenolic, flavonoid and antioxidant compounds is considered, collection of this plant from an altitude of 1700 meters is highly recommended.
Type of Study: Research |
Subject:
Special
Received: 2021/01/1 | Accepted: 2021/07/9 | Published: 2022/03/30
Received: 2021/01/1 | Accepted: 2021/07/9 | Published: 2022/03/30
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